This kit applies the principle of GICA to qualitatively detect the IgM and IgG antibody in human serum, plasma or whole blood. Take the test specimen and add it to the specimen well of the test paper, and then add 2 drops of specimen diluent. The IgM/IgG antibody in the specimen binds to the colloidal gold-labeled recombined antigen on the binding pad to form a complex, which diffuses forward along the nitrocellulose membrane (NC membrane) under chromatography. Then, it binds to an anti-human IgM/IgG antibody fixed on the NC membrane test line (M/G line) to form a purple-red band, showing the IgM/IgG antibody. The deeper the color of the band on the M/G line, the higher the concentration of the IgM/IgG antibody in the specimen will be. To monitor the effectiveness of the test card, a quality control line (C line) is set. Regardless of whether there is a M/G line, the C line shall show color development, or the test result will be judged as invalid.
By multiplex PCR-fluorescent probe technology, combined with fast one-step RT-PCR technology, the kit uses ORF1ab and the specific conserved sequence of nucleocapsid protein N gene as target regions. With the designed dual-target gene detection primer probe, the kit works with a high-efficiency RT-PCR reaction system for detection. After the end of the reaction, the results will be determined through analysis of the cycle threshold (Ct) of each channel. Moreover, the kit is designed with human housekeeping gene RNaseP for monitoring sampling, extraction,specimen addition, amplification and other related processes as an internal standard to effectively prevent false positive and false negative results, so as to ensure the specificity and accuracy of the test results.
The kit shall be stored at 2~30ºC and is valid for 18 months. After unpacking the aluminum foil bag, please use it as soon as possible.
Please read the Instructions for the kit carefully before use. The test result shall be determined within 15min after the specimen is added, or the result will be invalid.
